|
KMID : 0350519940470010527
|
|
Journal of Catholic Medical College
1994 Volume.47 No. 1 p.527 ~ p.539
|
|
|
Analysis of DNA Proliferative Variables and p53 protein Expression in non-Hodgkin's Lymphomas
|
|
|
|
|
Abstract
|
|
|
In non-Hodgkin's lymphomas, it has been well known that the prognosis of the disease depends on the clinical stage and therapeutic modality as the pathological grade. The ploidy and the synthetic phase fraction in cell cycle analysis were closely
related with pathological grade. Also, p53 is a tumor suppressor gene, located arm of chromosome 17, which encodes for a nuclear protein involved in the cellular growth control, regulating the entry of the cell into the S-phase. P53 mutation has
been
identified in a progressively increasing number of human malignancies, including non-Hodgkin's lymphoma.
This study was performed to evaluate the relationship of DNA ploidy pattern by flow cytometry, p53 protein expression and the pathologica grade.
Paraffin embedded tumor samples from 63 non-Hodgkin's lymphomas, 15 reactive hyperplasia of lymph nodes and 5 palatine tonsils as control group were analyzed by DNA flow cytometry and immunoperoxidase staining for p53 expression.
DNA proliferative fractions were calculated by bitmap gating using flow cytometry in S, G2, M phases and aneuploidy was determined from the DNA histogram using linear S-phase method. P53 protein expression was studied by immunoperoxidase staining
for
two different monoclonal antibodies, Pab 1810 (AB 2) and Pab 240 (AB 3).
Possible relationships between two indices (DNA proliferative variables and p53 protein expression rate) and histologic grading were investigated in the non-Hodgkin's lymphomas.
@ES The results were summarized as follows:
@EN 1. The synthetic phase fractions of non-Hodgkin's lymphomas were 18.5±10.5%. Synthetic phase fraction was significnatly increased according to the pathologic grade of the lymphomas: 14.21% in low grade lymphoma, 18.66% in the intermediate
lymphoma
and 23.53% in high grade lymphomas (P=0.0273).
2. 66.67% of high grade lymphoma, 50.00% of intermediate grade lymphoma and 15.38% of low grade lymphoma showed aneuploidy pattern in DNA flow cytometry. The differences of frequency of aneuploidy between low grade, intermediate grade and high
grade
ymphomas were statistically significant(P<0.0001).
3. p53 protein was present in 39.68% of the non-Hodgkin's lymphomas with the monoclonal antibody PAb 1801 (AB 2) and in 22.22% with the monoclonal antibody PAb 240(AB 3).
4. There was a positive correlation between PAb 1801 (AB 2) expression rate and pathological grade (X*=12.882 p<0.005). Positive stainings of PAb 1801(AB 2) were in 30.77% of low grade lymphoma, 38.24% of the intermediate grade lymphoma and
50.00%
of
the high grade lymphoma.
5. There was a negative correlation between PAb 240(AB 3) expression rate and pathologic grade. The positive staining of PAb 240 (AB 3) were in 30.77% of low grade lymphoma, 20.59% of the intermediate grade lymphoma and 18.75% of the high grade
lymphoma.
6. Tissues from 15 cases of reactive hyprerplasia and 5 cases of palatine tonsils were negative for p53.
7. There was a positive correlation between PAb 1801(AB 2) expression and aneuploidy(X*=3.755 P=0.0477).
8. There were no correlation between p53 protein expression and synthetic phases fractions.
In conclusion, these suggest that the parameters of flow cytometric analysis such as DNA aneuploidy and synthetic phase fraction may be correlated with pathological grade. P53 protein expression may play a part in the development of non-Hodgkin's
lymphomas and correlates the pathological grades of non-Hodgkin's lymphoma. The concentration of p53 protein positivity in high grade lymphomas suggests that p53 protein may be involved in the transformation of low grade lymphoma to more
aggressive
types.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|